Preeclampsia which happens in 5-10% of pregnancies is associated with maternal and prenatal morbidity and mortality in all over the world. Preeclampsia leads to high rates of preterm birth, small for gestational age infant. The objective of the present study was to evaluate the effects of paternal cigarette smoking before and in conception on risk of preeclampsia in non smoker women without exposure to second-hand tobacco smoke. In this cohort study, 302 healthy pregnant nonsmoker women were entered. They were divided in two groups with non smoker husband (n=158) and smoker husband (n=144) in conception time. All of women reported no exposure of cigarette smoking before and in pregnancy. Rate of preeclampsia compared in two groups. Rate of preeclampsia is significantly higher in pregnant nonsmoker women that their husbands were smoker. (P=0.030) , OR= 2.808 [ CI 95% 1.364 5.781]. Paternal smoking in three months before until time of conception is a risk factor for preeclampsia.