— Soil sample was collected from the vicinity of the rice and chick pea plants. Forty four bacterial strains and seven strains of actinomycetes had been isolated. Among forty four strains, one strain was Serratia and four strains were Azotobacter species due to their distinct colonial morphology, microscopic morphology and biochemical characterization. From the study on each fertilizing activity, many effective microbial isolates, four strains as nitrogen fixer, six strains as P-solubilizer, ten K-decomposing strains, were occurred. In P-solubilizing activity, Serratia was the most efficient one, having 35 mm in zone diameter on plate screening and 750.88 ppm by UV spectrophotometric measurement. In quantitative analysis by AAS, the k-decomposing strain defined as K23 could solubilize 10.46 ppm and strain K11 could solubilize 9.92 ppm respectively. In the study on N2 fixing activity of Azotobacter species by ammonium test kit, A1 and A4 could fix nitrogen from 0.2 to 0.5%. The selected strains had co-existence on one another and so these strains were studied in field trial application on rice cultivation. The panicle length, seed number per panicle, 1,000 seed weight and estimated yield of the rice plot which use biofertilizer was greater than the others which were splashed with organic fertilizer and urea. The estimated rice yields from the use of biofertilizer, organic fertilizer and urea were 100, 70 and 80 baskets/acre respectively.