This study has been devoloped and validated a quick method for confirmatory analysis of nitrofuran metabolites according to the European Commission Decision 2002/657/EC requirements. The quantitative and confirmatory determination of nitrofuran metabolites was performed by liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS] in the positive ion mode. An in-house method validation was performed and the data reported on specificity, linearity, recovery, CCα and CCβ. The advantage of this method is that avoids the use of clean-up by SPE (Solid-Phase Extraction] and shortens incubation period for derivatisation with nitrobenzaldehyde in overnight or 16 h in the 37 ºC temperature in the dark and then low levels of nitrofuran metabolites are detectable and quantitatively confirmed at a rapid rate in egg samples.