Fuels derived from conventional fossil resources have been the predominant form of energy for a few decades. However, it has been observed that fossil fuel availability would eventually run out. The diminishing supply of conventional fossil fuel reserves, their adverse effects on the ecosystem, which includes the formation of harmful gases and pollutants, together with worries about the economy and energy demand, have prompted the quest for a new, sustainable, readily available, inexpensive, environmentally friendly, and renewable energy source to replace the presently available petro-diesel fuel. Vegetable oil has been found to be one of the potential alternatives for neat diesel in the aforementioned scenario. However, the primary drawbacks of these oils are their extremely high viscosity, poor volatility, and associated problems that arise from employing them for a prolonged duration of time in engines. These challenges can be resolved by converting these vegetable oils into alkyl esters. The alkyl esters derived from vegetable oil are termed biodiesel, the best suitable substitute for petro-diesel owing to their renewable and low-emission facilities. Due to the high biodegradability and non-existent toxic substances, biodiesel from renewable sources such as animal fats, edible and non-edible oils, and algae has become a popular replacement for conventional petro-diesel. In the meantime, feedstock alone costs approximately 60 to 70 % of the cost required for biodiesel production. As a result, identifying the appropriate, affordable, and effective feedstock is essential for the continual production of biodiesel with minimum process cost. Subsequently, the cost of production will be drastically reduced when utilising inexpensive, non-edible oils, spent cooking oil, animal fats, and algae as feedstock for producing biodiesel. Raphanus sativus seeds, which are non-edible and have not yet been thoroughly investigated for their potential use as a feedstock for biodiesel, are the source of the biodiesel used in this study. Oil from seeds is extracted using a mechanical expeller, and the yield was determined to be 46.2±2 weight percent. The physicochemical properties were then examined using the standard AOAC procedures. The current work used a catalytic transesterification reaction to produce the biodiesel. The parameters influencing the biodiesel production processes, like Methanol to Oil ratio, catalyst, process temperature and process time, must be optimised for better process yield. To investigate the consequences of the influencing parameters and to optimise the biodiesel yield, the Taguchi statistical approach, Response Surface Methodology, and Analysis of Variance table were employed, and the outcomes were compared. The results indicate that the Taguchi method gave the results comparably with the RSM method in a limited set of experimental runs. At the optimised condition, methanol to oil molar ratio 9:1, catalyst concentration 1 wt%, reaction temperature 50 °C and reaction time 30 min, biodiesel yield was 94.58 wt%.