Indian medicinal plants have a traditional background that they have potential to use as antimicrobial agents. Pedalium murex showed broad spectrum antimicrobial activity against three fungal strains Aspergillus niger, Aspergillus flavus, Candida albicans and five bacterial strains Escherichia coli, Staphylococus epidermis, Klebseilla pneumonia,Citrobactor diverses, Enterococus faecalis.The Ethanolic extracts were tested against selected test bacteria and fungi through disc diffusion assay where amoxicillin was used as standard. The results showed that alcoholic extract possess good antimicrobial activity against selected test bacteria and fungi. The present results therefore offer a scientific basis for traditional use of the various extract of Pedalium murex.